Composite
Part:BBa_K1378005:Design
Designed by: Meng Liuyi & Zhang Zijian Group: iGEM14_Peking (2014-10-03)
INPNC-linker2-MVN-linker1-mRFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 466
Illegal AgeI site found at 1915
Illegal AgeI site found at 2027 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1029
Design Notes
We optimized the sequence of MVN beforehand. We added a 7aa linker between INPNC and MVN which can be cut by rTEV protease, and there is also a 10aa flexible protein domain linker between MVN and INPNC to help the two domains function normally.
Source
This is a composite part so we constructed it using various different smaller parts. INPNC comes from the existing part BBa_K523013. The sequence of MVN can be found in the genomic sequence of Microcystis aeruginosa PCC7806 and the gene was synthesized by a company (sequence can also be found in the part BBa_K1378003). mRFP is common and we took it from the vector J61002.