Composite

Part:BBa_K1378005:Design

Designed by: Meng Liuyi & Zhang Zijian   Group: iGEM14_Peking   (2014-10-03)

INPNC-linker2-MVN-linker1-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal AgeI site found at 1915
    Illegal AgeI site found at 2027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1029


Design Notes

We optimized the sequence of MVN beforehand. We added a 7aa linker between INPNC and MVN which can be cut by rTEV protease, and there is also a 10aa flexible protein domain linker between MVN and INPNC to help the two domains function normally.


Source

This is a composite part so we constructed it using various different smaller parts. INPNC comes from the existing part BBa_K523013. The sequence of MVN can be found in the genomic sequence of Microcystis aeruginosa PCC7806 and the gene was synthesized by a company (sequence can also be found in the part BBa_K1378003). mRFP is common and we took it from the vector J61002.

References